| 초록 |
The development of a rapid and simple detection method for nucleic acid anlaytes is crucial for diagnosis of a disease and subsequent treatment. Herein, we designed a simple assay based on the production of amplified target nucleic acids introduced with rapid clickable functionalities. The assay was performed by synthesis of rapid clickable DNA of the specific targets by PCR thermal cycling using a mixture of triphosphate (dATP, dTTP, dGTP, and C8-Alkyne-dCTP) to obtain the alkyne-modified DNA. The amplified target DNA was detected by adding multi-arm-PEG-azide for Cu(Ⅰ)-catalyzed azide-alkyne cycloaddition reaction (CuAAC) and inducing hydrogel formation. The gelation of multi-arm-PEG in presence of clickable target DNA was characterized by visualization with the naked eye as well as rheological analyses. This assay could be a useful and easily applicable diagnostic tool for various diseases such as infections, cancer, and genetic disorders. |
