| 초록 |
Poly(catecholamine) is well-known universal adhesive used in material independent surface chemistry. The chemical structure of poly(catecholamine) resembles that of melanin, a class of natural pigments that generate the variation in color of skin, hair, and eye in human. Inspired by the similarity of these synthetic and natural organic materials, we herein designed an enzyme-initiated colored adhesive platform to detect biomarkers via naked-eye. Due to their unique adhesiveness characteristic, colored adhesive provides the visible signal only where the targeted biomarker is detected via enzyme-linked antibodies. Therefore, it allows highly concentrated pigments unlike conventional substrates used in colorimetric assays. Colored adhesive-based ELISA-type bioassay of a model biomarker, α-fetoprotein (AFP), showed 3 ng/mL of detection limit by spectrophotometer and 32 ng/mL of detection limit by naked-eye. |
