| 초록 |
Agmatine-containing bioreducible polymer (poly(CBA-AG)) was synthesized for non-viral gene delivery systems, which take advantages of the biodegradability of disulfide bonds and the cell-penetrating ability of guanidine groups. In agarose gel electrophoresis, poly(CBA-AG) was able to retard pDNA from a weight ratio of 2 but pDNA was released from polyplexes even at a weight ratio of 20 in reducing condition (2.5mM DTT) due to their degradation. It could condense pDNA into positively charged (30-40mV) and nano-sized particles (200-300nm). The cytotoxicity of poly(CBA-AG) was generally low but somewhat higher than unmodified backbone polymer, poly(CBA-DAB) due to the strong charges of guanidine groups. It could transfect both HeLa and Neuro2a cells more efficiently than poly(CBA-DAB) and showed even higher transfection efficiency than PEI25k, especially in Neuro2a cells. Thus, poly(CBA-AG) can be a promising polymer for efficient gene delivery systems. |
