화학공학소재연구정보센터
학회 한국화학공학회
학술대회 2008년 봄 (04/23 ~ 04/25, 제주ICC)
권호 14권 1호, p.519
발표분야 생물화공
제목 Purification of Aspergillus niger Xylanase Produced in Solid State Fermentation
초록 Xylanase catalyzes the hydrolysis of the xylan backbone to xylooligosaccharides. A variety of microorganisms, including bacteria, yeast and filamentous fungi, have been reported to produce xylanase. For the production of enzyme, solid state fermentation (SSF) offers advantages over submerged fermentation (SmF). In this study, xylanase was produced by A. niger KK2 in SSF with rice straw and extracted with buffer and then concentrated by ultrafiltration. Xylanase was purified by ion-exchange chromatography methods in FPLC system. Purified xylanase was characterized and its apparent molecular weight was about 24 kDa, as estimated by SDS-PAGE.
저자 신현석1, 강성우1, 송윤석1, 이종호1, 신현용1, 이진영1, 박철환2, 김승욱1
소속 1고려대, 2광운대
키워드 Xylanase; purification
E-Mail
원문파일 초록 보기