| 초록 |
Recently, protein delivery systems have been extensively investigated for treatment of ischemic diseases as an alternative to surgical operations. One of the key proteins for neovascularization is the vascular endothelial growth factor(VEGF) that promotes migration, proliferation and differentiation of endothelial cells. However, intra-arterial injection or direct administration into ischemic tissues has been extremely limited due to its short lifetime in the body. We hypothesized that controlled delivery of VEGF could be achieved for a prolonged time period using a combination of alginate gels and PLGA microspheres, and this combination system could be useful for therapeutic angiogenesis in vivo. We found that the sustained release of VEGF from the system was achieved for three weeks and bioactivity of the released VEGF was maintained in vitro. The number of newly formed arteriole and capillary increased when the combination system was implanted into the ischemic hindlimb of mice. |
