| 학회 |
한국고분자학회 |
| 학술대회 |
2009년 봄 (04/09 ~ 04/10, 대전컨벤션센터) |
| 권호 |
34권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Quantifying cell-material interactions using FRET technique |
| 초록 |
Alginate was chemically conjugated with adhesion ligands (e.g., G4RGDASSK), and RGD island spacing in the gel was varied from 36 to 78 nm while the average bulk ligand density was kept constant. We found that the mechanical properties of the gels were not altered by introducing ligands in the gels. The strength of cell adhesion was quantified using fluorescence resonance energy transfer (FRET) technique and cell adhesion index (CAI). FRET is a technique that can quantify the amount of energy transfer from donor to acceptor molecule. CAI was used to assess the relative number of cells remaining on the gel after application of centrifugal forces. Both methods have shown the same trend related to cellular adhesion onto the ligand-organized gels. These results support that biophysical cues such as ligand organizations on synthetic extracellular matrices play an important role to modulate cellular responses to its microenvironment. |
| 저자 |
Munira B. Shahbuddin, 박홍현, 이재원, 이근용
|
| 소속 |
한양대 |
| 키워드 |
alginate; hydrogel; FRET; interactions; ligand spacing; ligand organigation
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| E-Mail |
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