| 학회 |
한국화학공학회 |
| 학술대회 |
2019년 봄 (04/24 ~ 04/26, 제주국제컨벤션센터) |
| 권호 |
25권 1호, p.357 |
| 발표분야 |
생물화공(Biochemical Engineering) |
| 제목 |
Application of Engineered Escherichia coli for Secretory Heme Production |
| 초록 |
Heme has been considered as one of the important bioproduct with its wide usage in healthcare and food supplement. Here we demonstrate production of free heme by secretion using engineered E. coli strains, through the C5 pathway with optimized downstream pathway for heme biosynthesis. Furthermore, inactivation of several genes encoding potential heme-degrading enzymes showed a result of 7.88 mg/L of total heme with 1.26 mg/L of extracellular portion in flask cultivation. Fed-batch fermentations of strain with heme exporter CcmABC overexpression from glucose only and glucose supplemented with L-glutamate secrete 73.4 and 151.4 mg/L of heme, respectively, which takes 63.5% and 63.3% of total heme produced for each. The engineered E. coli strain presented here would be valuable for microbial production of free heme. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries (Grants NRF-2012M1A2A2026556 and NRF-2012M1A2A2026557) from the Ministry of Science and ICT through the National Research Foundation (NRF) of Korea] |
| 저자 |
박다현, Xin Rui Zhao, 최경록, 이상엽
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| 소속 |
한국과학기술원 |
| 키워드 |
생물화학공학 |
| E-Mail |
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| 원문파일 |
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