| 학회 |
한국공업화학회 |
| 학술대회 |
2016년 봄 (05/02 ~ 05/04, 여수 엑스포 컨벤션) |
| 권호 |
20권 1호 |
| 발표분야 |
생체재료_포스터 |
| 제목 |
Simple and Rapid Aptasensor Rolling Circle Amplification for Detection of Middle East Respiratory Syndrome |
| 초록 |
Middle East respiratory syndrome (MERS) is a viral respiratory disease caused by a coronavirus (MERS‐CoV). Following WHO indication, a simple and robust microfluidic array chip assay has been developed based on RCA of adaptor templates upon the hybridization of MERS pathogens. Long single strands of RCA products can be visualized by SYBR gold staining. This assay shows high sensitivity and potent ability to detect various disease according to design of DNA template. Furthermore, this assay doesn’t need reverse transcription step and all process is at room temperature. It makes special equipment such as thermal cycler unnecessary contrast to PCR method. It is likely that the development of this facile and sensitive MERS pathogen detection assay may relieve excessive workloads. |
| 저자 |
정일영, 이혁진
|
| 소속 |
이화여자대 |
| 키워드 |
Rolling circle amplification(RCA); DNA/RNA pathogens; Middle East Respiratory Syndrome(MERS); Diagnosis
|
| E-Mail |
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