| 학회 |
한국화학공학회 |
| 학술대회 |
2008년 가을 (10/23 ~ 10/24, 부산 BEXCO) |
| 권호 |
14권 2호, p.2750 |
| 발표분야 |
생물화공 |
| 제목 |
Improved succinic acid production based on proteomic and physiological analysis of Mannheimia succiniciproducens |
| 초록 |
A genetically engineered succinic acid overproducing mutant, M. succiniciproducens LPK7, was also developed based on full genome sequence. In this study, 2-DE and tandem mass spectrometry were used to analyze proteome of the mutant cells at the exponential and stationary phases. These results were compared with those of the wild type strain (MBEL55E) to elucidate the global physiological and metabolic changes responsible for succinic acid overproduction. Several features including expression level changes of pyruvate metabolism associated enzymes, NAD(P)H-dependent enzymes, and ATP-dependent enzymes were described in detail. Based on these proteome analyses, we finally improved the succinic acid production. [This work was supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MOST) (2005-01294). Further supports by the LG Chem Chair Professorship, IBM SUR program, Microsoft, and by the KOSEF through the Center for Ultramicrochemical Process Systems are appreciated.]. |
| 저자 |
이정욱1, 송효학2, 최 솔3, 유종신1, 김지만2, 이상엽3
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| 소속 |
1Metabolic and Biomolecular Engineering National Research Laboratory, 2Department of Chemical & Biomolecular Engineering and BioProcess Engineering Research Center, 3KAIST |
| 키워드 |
Mannheimia succiniciproducens; proteome; succinic acid
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| E-Mail |
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| 원문파일 |
초록 보기 |
