| 초록 |
Gene delivery system using peptide micelles has received a considerable amount of attention. Peptide micelle system presents relatively low cytotoxicity and transfection efficiency than other vector system. In this report, we propose a micelle model overcoming poor transfection efficiency. The micelle introduces GFLG sequence, arginine and histidine. GFLG(Gly-Phe-Leu-Gly) sequence help escape from endosome-lysosome compartment by enzymatically cleavable characterization. The sequence is known to be cleaved by enzyme Cathesin-B. Ariginine is a key element of the cell-penetrating peptide and histidine display enhanced proton sponge effect. To form micelle, we make amphiphilic units composed of peptides having different property. The GFLG sequence is a hydrophobic core. The Hn-R segment is hydrophilic and cationic head group. In results, micelle/pDNA complex formation was analyzed by Agarose gel. The transfection efficiency and cytotoxicity were measured by luciferase assay and MTT assay. |
