| 초록 |
Saccharomyces cerevisiae sake kyokai No. 6 strain(S. cerevisiae sake K6) can accumulate remarkably higher level of S-adenosyl-L-methionine(SAM) compared to wild type Saccharomyces cerevisiae. However, DNA recombinant technologies to improve property of S. cerevisiae sake K6 have been trapped because of absence of selection marker. In our study, we developed leucine auxotrophic mutant of S. cerevisiae sake K6 strain through UV mutagenesis. This auxotrophic mutant strain(K6-1) showed similar growth rate and SAM productivity. Futhermore, we introduced plasmid contains Sam2 gene, which encodes SAM synthase, to the auxotrophic mutant strain. This recombinant sake yeast strain can accumulate SAM about 50% of its dry cell weight, which is almost maximum amount in single yeast cell. |
