| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 봄 (04/12 ~ 04/13, 제주 ICC) |
| 권호 |
32권 1호 |
| 발표분야 |
나노바이오기술용 고분자(의료용 고분자 부문위원회) |
| 제목 |
Controlled bio-conjugation between polymers and proteins |
| 초록 |
One method to bio-conjugate proteins with polymers is to use end-functionalized polymers which have specific reactivity or interaction with targeted proteins. In my presentation, a new approach to bio-conjugate proteins and polymers will be discussed. The developed method is based on the strong interaction with a sequence of histidine residues (His-tag) in genetically engineered proteins and Ni2+ complexed nitrilotriacetic acid (NTA) group at the polymer chain end. In our study, green fluorescent protein (GFP) with His-tag was conjugated with Ni2+-NTA end-functionalized polystyrene. His-tagged GFP was expressed in recombinant E. coli BL21 (DE3) and end-functionalized polystyrene was prepared by Atom Transfer Radical Polymerization using NTA containing initiator. As His-tagged proteins is well-known to have specific binding affinity with Ni2+-NTA, His-tagged GFP was able to be conjugated with Ni2+-NTA end-functionalized polystyrene in a controlled fashion. |
| 저자 |
백현종 |
| 소속 |
부산대 |
| 키워드 |
Bio-conjugation; Ni-NTA; Histidine-tag; Polystyrene; ATRP
|
| E-Mail |
|
