| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 봄 (04/12 ~ 04/13, 제주 ICC) |
| 권호 |
32권 1호 |
| 발표분야 |
나노바이오기술용 고분자(의료용 고분자 부문위원회) |
| 제목 |
Controlled Bioconjugation of Poly(poly(ethylene glycol)methyl ether methacrylate)with Green-Fluorescent Proteins |
| 초록 |
We developed a new approach to bio-conjugate proteins and polymers. The developed method is based on the strong interaction with a sequence of histidine residues (His-tag) in genetically engineered proteins and Ni2+ complexed nitrilotriacetic acid (NTA) group at the polymer chain end. In our study, green fluorescent protein (GFP) with His-tag was conjugated with Ni2+-NTA end-functionalized poly(ethylene glycol) methyl ether methacrylate. His-tagged GFP was expressed in recombinant E. coli BL21 (DE3) and end-functionalized poly(ethylene glycol)methyl ether methacrylate was prepared by Atom Transfer Radical Polymerization using NTA containing initiator. As His-tagged proteins is well-known to have specific binding affinity with Ni2+-NTA, His-tagged GFP was able to be conjugated with Ni2+-NTA end-functionalized poly(ethylene glycol)methyl ether methacrylate in a controlled fashion. |
| 저자 |
이효경, 조홍열, 김유진, 이선구, 백현종
|
| 소속 |
부산대 |
| 키워드 |
ATRP; end-functionalized polymer; bioconjugation; His-tagged protein; Ni2+-NTAcomplex
|
| E-Mail |
|
