| 초록 |
Patterned living cell-based models potentially provide functional information for the monitoring of cell-cell, cell-drug and cell-biomaterial interactions. We created complex living cell patterns via surface engineering with a combined use of piezoelectric inkjet printing and a membrane-anchoring polymer. Non-adherent cells (SNU-620) as well as adherent cells (HeLa) were stably anchored and patterned on a glass substrate through interactions between cell membranes and the cell membrane anchoring polymer, which is composed of an oleyl group and a poly(ethylene glycol) (PEG). The effects of an apoptosis inducer (staurosporine) and an anticancer drug (paclitaxel) were visualized on cells immobilized on the polymer-printed patterns. This approach could be potentially applied to various biomedical technologies which require precisely controlled living cell patterns, such as cell-based assays, biosensors, biochips, and tissue engineering. |
