| 초록 |
The recent rapid rise in oil prices, Biomass using bio-fuel (bio-diegel, bio-ethanol, bio-gas, etc.) production has attracted considerable attention. In the present study, Electron beam induced mutant generation was tried using a photosynthetic microalgae, Nannochloropsis oculata (N. oculata), for the production of biodiesel. The resulting colonies were isolated and further cultured in liquid f/2 medium. After a few weeks after cultivation, cell growth, dry cell weight, protein, chlorophyll, carotenoid and lipid content were measured. Two mutants among thousands colonies showed increased cell growth and chlorophyll biosynthesis and high lipid accumulation as compared to wild type. It was also observed that the increased cell growth rate is associated with the overexpressed intracellular proteins. |
