| 초록 |
For the early detection of severe disease in every world, demands on fast and cheap detection methods of protein disease markers in biomedical science are increasing greatly. However, while current protein detection methods such as HPLC, and Western blotting are sensitive enough, show limitations in point-of-care testing. Several studies using fluorescence nanoparticles to overcome this limitation are ongoing, but still need more time. In this research, we introduce a simple and fast assay using carbon dots with fluorescence which can be switched on and off depending on the presence of the protein target. The assay just required the mixing of the carbon dot solution with dopamine along with the solution including the target. Fluorescence signal turned off with polydopamine formation while the addition of a target protein caused signal to turn on. With further research, method can be applied as a simple and rapid diagnostic assay for ubiquitous detection of various protein targets. |
