| 학회 | 한국고분자학회 |
| 학술대회 | 2004년 가을 (10/08 ~ 10/09, 경북대학교) |
| 권호 | 29권 2호, p.391 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Cell Adhesion Behaviors of Peptide Ligand-grafted Biodegradable Porous Scaffolds for Tissue Engineering |
| 초록 | The interaction between the scaffold surfaces and specific cells play an crucial role in tissue engineering applications. Recently, some synthetic bioactive ligands such as the peptide Arg-Gly-Asp (RGD) have been immobilized into polymeric materials to improve specific biological activity. In this study, biodegradable porous poly(L-lactic acid) (PLLA) scaffolds were prepared by using gas foaming method for unipore and a gas foaming and phase-separation methods for dual pore scaffolds (Fig. 1). The obtained biodegradable unipore and dual pore PLLA scaffolds with hydrophobic surfaces were activated by using plasma treatment for hydrophilic surface. Some carboxylic acids on PLLA surfaces were obtained by acrylic acid (AA) grafting and then readily reacted with GRGD and GRDG.1 The properties of porous PLLA scaffolds were measured by water absorption, SEM, and mechanical test. In addition, the interaction of some cells with ligands-grafted porous scaffolds were evaluated by SEM, WST-1 assay, GAG, and collagen contents as a function of surface peptide density. The surface of PLLA scaffolds was more hydrophilic after AA and ligand grafting but their surface morphologies were relatively similar. The order of cell adhesion on the porous PLLA scaffolds was PLLA control ＜ PLLA-g-PAA＜ PLLA-g-GRDG ＜ PLLA-g-GRGD (Fig. 2). These biodegradableporous PLLA scaffolds are useful for intelligent matrix to regenerate artificial tissues and organs. Fig. 1. RGD-grafted unipore and dual pore PLLA scaffolds. PLLA control PLLA-g-PAA PLLA-g-GRDG PLLA-g-GRGD Fig. 2. SEM morphologies of dual pore PLLA scaffolds (Fibroblast culture for 12 h). Reference 1. M. Gümüşderelioglu and H. Turkoglu, Biomaterials, 23, 3927 (2002). |
| 저자 | 정현정1, 안광덕1, 안동준2, 한동근1 |
| 소속 | 1한국과학기술(연), 2고려대 |
| 키워드 | tissue engineering; ligand peptide; scaffold; porosity |
