| 학회 | 한국고분자학회 |
| 학술대회 | 2002년 가을 (10/11 ~ 10/12, 군산대학교) |
| 권호 | 27권 2호, p.189 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Evaluation of cells on polymer surface with different wettability by RT-PCR analysis |
| 초록 | The adhesion and proliferation of mammalian cells on polymeric biomaterials depends mainly on the surface characteristics such as wettability, chemistry, charge, roughness, and rigidity. A large number of research groups have studied the interaction of cultured cell and various polymers with different wettabilities to correlate the relationship between surface wettability. In this study, we have recognized that adhesion and proliferation of cells on polyethylene (PE) films depended on the surface properties mainly focused on analyzing the RT-PCR pattern of expression exhibited by several growth-associated genes. Plasma treatment of PE films was developed to increase surfaces wettability and to improve adhesion and proliferation of NIH/3T3 fibroblasts. The water contact angle of the film surfaces gradually decreased with increase of plasma treatment time. The surface-modified PE films were characterized by the measurement of the water contact angle and ESCA. The adhesion and proliferation of cells on PE films with different wettability were characterized by SEM, cell count, and RT-PCR. Analyzing the pattern of expression was compared with several growth-associated genes (e.g., c-fos, c-myc, and p53) in NIH/3T3 fibroblasts onto PE surfaces with different wettability. Acknowledgment : This work was supported by MOWH (IMT-2000,01-PJ11-PG9-01NT00-0011). |
| 저자 | 박기숙1, 황혜진2, 김은정*3, 이상진**, 강길선, 이종문, 이해방** |
| 소속 | 1전북대, 2*전북대, 3**한국화학(연) |
| 키워드 | |
