| 학회 |
한국화학공학회 |
| 학술대회 |
2017년 봄 (04/26 ~ 04/28, ICC 제주) |
| 권호 |
23권 1호, p.457 |
| 발표분야 |
생물화공 |
| 제목 |
Production of 3-Aminopropionic acid, a Precursor for Nylon-3, by Metabolically Engineered Escherichia coli |
| 초록 |
3-aminopropionic acid (3-AP) is an important platform chemical for manufacturing acrylamide and acrylonitrile. Using a previously developed fumaric acid producing E. coli strain as a host, the C. glutamicum panD gene (encoding L-aspartate-α -decarboxylase) was overexpressed and the native promoter of the aspA gene was replaced with the strong trc promoter. The aspA and phosphoenolpyruvate carboxylase (ppc) genes were additionally overexpressed, and the ammonium sulfate was supplemented in the medium, which resulted in the production of 3.49 g/L 3-AP. Optimization of PPC expression level by using synthetic promoter and RBS sequences and replacement of native promoter of the acs gene led to produced 32.3 g/L of 3-AP in 39h with an overall yield and productivity of 0.135 g 3-AP/g glucose and 0.828 g/L/h, respectively, in fed-batch fermentation (This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Science, ICT and Future Planning (MSIP) through the National Research Foundation (NRF) of Korea (NRF-2012M1A2A2026556 and NRF-2012M1A2A2026557)). |
| 저자 |
김제웅1, 송찬우2, 이종민3, 고유성1, 이상엽1
|
| 소속 |
1KAIST, 2GS Caltex, 3CJ 제일제당 |
| 키워드 |
생물화공 |
| E-Mail |
|
| 원문파일 |
초록 보기 |
