| 학회 |
한국재료학회 |
| 학술대회 |
2014년 봄 (05/15 ~ 05/16, 창원컨벤션센터) |
| 권호 |
20권 1호 |
| 발표분야 |
D. 바이오 재료(Biomaterials) |
| 제목 |
Fabrication of Hydroxyapatite(HAp) granules loaded Oxidized Alginate-Gelatin-Biphasic Calcium Phosphate(Oxg Alg-Gel-BCP) hydrogel complex and its in-vitro evaluation for bone tissue regeneration |
| 초록 |
There are many reports on the fabrication of injectable bone substitutes which are combined with bio-ceramics and bio-polymer for bone tissue engineering. In this work, we fabricated spherical HAp granules and loaded into Oxidized Alginate-Gelatin-Biphasic Calcium Phosphate in order to use as a bone substitute system. For the fabrication of HAp granules, PVA-Gel-HAp composite slurry was dropped first by a syringe through the nozzle on liquid nitrogen maintaining the flow rate, distance of dropping and then lyophilized for 2 days. Then polymers were burnt out from HAp granules by tube furnace and finally sintering occurred at 13500 C. These HAp granules were loaded into Oxg Alg-Gel-BCP slurry and freeze-dried for 2 days to obtain porous structure. Detailed morphological and material characterizations such as microstructure analysis, compressive strength, porosity, FTIR spectra were carried out. MTT assay, confocal imaging and live-dead assay were carried out as in vitro experiments to observe the cell attachment, proliferation with Rat Bone Marrow Mesenchymal Stem Cell (RBMSC). |
| 저자 |
Avik Sarker1, Kallayanashish Paul2, Min Young Ki3, Byong Taek Lee4
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| 소속 |
1Department of Regenerative Medicine, 2College of Medicine, 3Soonchunhyang Univ., 4Cheonan 330-090 |
| 키워드 |
granules; Oxidized Alginate; Gelatin; BCP; liquid nitrogen
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| E-Mail |
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