| 초록 |
Potential of riboswitch which implements cis-regulatory mechanism has been highlighted for monitoring various molecules. Several parameters of riboswitch should meet the requirements to be applied in user-defined condition. However, tuning parameters of riboswitch such as dynamic range, fold activation, and operational range requires detailed information. Therefore, alternative simple tuning options are necessary. Here, we demonstrate three strategies for optimization of functional parameters using an artificial L-tryptophan riboswitch. We modulated the strength of the promoter or the copy number of the plasmid to optimize dynamic range and fold activation. Then tetA gene was utilized to amplify the response by tetracycline supplementation. Finally, a riboswitch variant was constructed using an aptamer with distinct affinity to L-tryptophan to adjust the operational range. All strategies could be easily applied to the optimization of other riboswitches owing to their simplicity. |
