| 초록 |
In this study, doxorubicin (DOX)-conjugated chitosan was synthersized by hydrazone bond. In addition, target ligand peptide (TL) was grafted to DOX-conjugated chitosan (TCD). The characters of TCD was analyzed by H-NMR, DLS, and HPLC. Cytotoxicity and antitumor activity was performed by MTT assay. Antitumor activity of TCD was higher than free drug and TL-free CD. Theses results suggest that the uptake efficiency of TCD into cancer cell was enhanced by receptor-mediated endocytosis and doxorubicin was easily released from TCD at late endosomal pH (pH 5.0) due to acidic pH-cleavable linkage. This research was supported by basic science research program through the national research foundation of korea (NRF) funded by the ministry of education. Science and technology (NRF-2012R1A2A2A01014898). |
