| 학회 |
한국화학공학회 |
| 학술대회 |
2020년 가을 (10/14 ~ 10/16, e-컨퍼런스) |
| 권호 |
26권 1호, p.71 |
| 발표분야 |
고분자 |
| 제목 |
colorimetric multiplex immunoassay using hydrogel microparticle |
| 초록 |
In accordance with growing demands for quantification of multiple proteins in solution, bead-based immunoassay using hydrogel microparticles has one of the method noted for its possibility to detect multiple analytes with graphycally-encoded microparticles. However, it still remains constraints for bead-based immunoassay using microparticles to be applied practically, because microparticles are labelled with fluorescent dye which has problem for fluorescence quenching and requres expensive equipments to analyze signal. Herein, we introduce colorimetric detection signaling method to bead-based assay. Using colour dyes generated in an amplifiable enzymatic reaction which accumulate in pores of microparticle in the presense of a target proteins, the hydrogel microparticles were imaged and anlaysed using low-cost portable devices. |
| 저자 |
김주연1, 노윤호1, 이현지2, 김현웅1, 김선민3, 봉기완1
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| 소속 |
1고려대, 2georgia tech, 3보라매병원 |
| 키워드 |
고분자 |
| E-Mail |
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| 원문파일 |
초록 보기 |
