| 초록 |
Versatile tumor models are available to study cancer biology and to screen anti-cancer drugs. Extracellular matrix (ECM) is the major components of engineered 3D tumor model, which promotes cancer proliferation and invasion by directly interacting with the cancer cell. However, ECM hydrogels, such as collagen-based hydrogel or Matrigel, do not reflect the heterogeneity of tumor microenvironment in cancer patients. In this talk, I present a the use of patient-derived extracellular matrix (pECM) and patient-derived glioma cancer cell (pGC) to reconstruct personalized microtumor model. By using the patient-derived microtumor, we investigated cell morphology, invasion, gene expression and drug response. Our results revealed that glioma cancer cells exhibit different migration strategies in various ECM environments. Thus, we suggest that tumor- and patient-specific 3D tumor models could provide a better understanding in tumor biology and guide to choose personalized therapeutic options. |
