| 학회 |
한국고분자학회 |
| 학술대회 |
2009년 가을 (10/08 ~ 10/09, 광주과학기술원 오룡관) |
| 권호 |
34권 2호 |
| 발표분야 |
고분자 나노 구조물의 합성, 분석 및 응용(국제심포지움) |
| 제목 |
Method Development for Direct Detection of Glycoproteins on the Aminophenylboronic Acid-Functionalized Self-Assembled Monolayers by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry |
| 초록 |
Glycosylation is one of the most abundant post-translational modifications of proteins and primarily associated with membrane-bound and secreted proteins. Glycoproteins fulfill a wide range of functions in the cell, and new functions are constantly being discovered. Therefore, the discovery and identification of glycosylated peptides and proteins, and the analysis of their glyco-structures are increasingly important in diagnosis and proteomics. This study describes a simple method for direct detection of glycoproteins after affinity-capturing on the plate using a combination of self-assembled monolayers(SAMs) and matrix-assisted laser desorption/ionization mass spectrometry(MALDI). The terminal hydroxyl groups of the SAMs were activated by N,N-disuccinimidyl carbonate, followed by conjugation with aminophenylboronic acid(APB). The formed APB-functionalized SAMs for MALDI plates were successfully applied to the affinity-capturing of a glycoprotein from the protein mixture. |
| 저자 |
장경국1, 김수현1, 이광섭2, 이경복1
|
| 소속 |
1한국기초과학지원(연), 2한남대 |
| 키워드 |
self-assembled monolayers; Glycoproteins
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| E-Mail |
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