| 학회 |
한국고분자학회 |
| 학술대회 |
2015년 봄 (04/08 ~ 04/10, 대전컨벤션센터) |
| 권호 |
40권 1호 |
| 발표분야 |
대학원생 구두발표 (영어발표, 발표15분) |
| 제목 |
Biomimetic peptide-grafted substrate for feeder-free culture of human pluripotent stem cells |
| 초록 |
Current culture methods for human pluripotent stem cell (hPSC) expansion require animal sources such as feeder cells or matrices derived from animals. Thus, it is difficult to obtain sufficient numbers of clinical grade hPSCs due to safety issues, quality control, and high expense. Here, we developed biomimetic peptide-grafted substrates for feeder-free, xeno-free culture of hPSCs. The biomimetic surface promoted specific integrin-mediated focal adhesion and cell-cell interactions, leading to enhanced adhesion, self-renewal, and pluripotency of hPSCs. Moreover, biomimetic peptides could be immobilized to support growth of hPSCs in a surface material-independent manner. The engineered substrates developed in this study provide a chemically defined, xeno-free culture system for clonal expansion and long-term maintenance of hPSCs. Acknowledgement: This work was supported by grants (grant numbers: NRF-2013R1A1A2A10061422 and HI14C1588). |
| 저자 |
박현지1, 양기석1, 이미현2, 전은제1, 장지호1, 김동욱1, 이해신2, 조승우1
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| 소속 |
1연세대, 2KAIST |
| 키워드 |
human pluripotent stem cells; polydopamine; vitronectin peptide; material-independent surface coating; feeder-free maintenance
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| E-Mail |
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