| 초록 |
In order to investigate the effect of culture pHs and aeration rates on poly(γ-glutamic acid)(γ-PGA) production, batch fermentation of Bacillus sp. RKY3 was carried out using modified E medium. Culture pHs between 5.0 and 8.0 were tested, and non-controlled pH experiment was also carried out. The maximum γ-PGA was obtained as 42g/L at non-controlled pH. Among the culture pHs tested, the highest molecular weight of γ-PGA was obtained as 85,000 at pH 7. During batch fermentation at non-controlled pH, the culture pH varied from 6.4 at 7.3. In addition, maximum dry cell weight and γ-PGA were obtained as 1.8g/L and 40g/L, respectively, at 0.5 vvm. Acknowledgement This study was financially supported by grant NO. R05-2003-000-10941-0 from the Korea Science and Engineering Foundation. |
