| 초록 |
Protein inactivation by reactive oxygen species (ROS) have been expected as cell death pathways from protein dysfunction; however, its detailed mechanisms and direct involvement in photodynamic therapy (PDT) have not been revealed and photosensitizers for PDT in low oxygen concentration have been limited. Herein, we present design strategy for efficient photosensitizers based on Ir(III) complexes and then report Ir(III) complexes that show superior ROS generation (1O2 quantum yield = 0.97) by both single and two photon activation, thereby serving as oxygen sensitive PDT agents for cancer cells by protein modifications (i.e., over-produced protein oxidation and heavy photo-crosslinking without any additives), especially localized on endoplasmic reticulum (ER) and mitochondria at their low concentrations (≤ 2 μM) with low energy light exposure (≤ 1 J cm-2). We also suggest plausible PDT pathways with protein dysfunctional modifications, which are characterized by mass spectrometry. |
