| 초록 |
The study of gene expression on a genomic scale is the most obvious opportunity to be made possible by a complete genome sequences of the model organisms. Recently developed DNA chip has emerged as a prime candidate for the performance of such analyses. DNA chip makes it possible to measure the transcription levels of all genes at the same time. High cell-density culture (HCDC) techniques for culturing E. coli have been developed for high-level production of various proteins and non-protein products such as amino acids and poly(3-hydroxybutyrate). Fed-batch cultures have most often been used to obtain high cell density. The most difficulty of process optimization by fed-batch culture is little knowledge on the physiology and metabolism during high cell density. Compared with low cell density, the cells are assumed to undergo large metabolic and physiological changes under high cell density (>50g DCW/L) culture condition. In this study, we focused on the effect of the high cell density on variation in metabolism and physiology of cell during fed-batch culture. |
