| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 봄 (04/10 ~ 04/11, 컨벤션 뷰로(대전)) |
| 권호 |
33권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Low molecular weight water soluble chitosan for accelerating DNA strand exchange |
| 초록 |
The association, dissociation and strand exchange of nucleic acids are essential processes in maintenance of living system. DNA strand exchange between double strand and its complementary single strand has a pivotal role in repair of damaged DNA and genetic recombination. In the previous study, we reported that the cationic comb-type copolymer, PLL-g-Dex showed higher efficacy on the accelerating DNA strand exchange. In this study, we investigated the accelerating effect of low molecular weight water soluble chitosan(LMWSC) on the DNA strand exchange reaction between a 20 bp duplex DNA and its complementary single strand. LMWSC has a high content of free amino groups in the chitosan chain, implying increased affinity with DNA. DNA strand exchange was studied by polyacrylamide gel electrophoresis(PAGE) using the duplex strand labeled with fluorescein isothiocyanate(FITC) and its complementary single strand. |
| 저자 |
이다미1, 고용기2, Biswajit1, 장미경3, 나재운3, 김원종1
|
| 소속 |
1포항공과대, 2인하대, 3순천대 |
| 키워드 |
DNA strand exchange; chitosan
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| E-Mail |
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