| 초록 |
As viral gene delivery systems are associated with severe security problems, nonviral gene delivery vehicles were developed. Natural and synthetic polycations displaying non-pathogenic and non-immunogenic properties have been employed as non-viral vectors for gene delivery due to the safety concerns. The main obstacle in gene therapy is the development of efficient, non-toxic delivery carriers, which effectively transport DNA inside the cell nucleus of the target. Cationic poly(aminoacids) like poly-L-lysine (PLL) are known to be efficient in condensing plasmid DNA into compact structure and have been used for in vitro and in vivo delivery of therapeutic DNA. To enhance it’s cell uptake ability deoxycholic acid (DOCA) is introduced to PLL. DOCA-PLL was synthesized through the ring-opening polymerization of N-(Z)-lysine-N-carboxyanhydride using N-deoxycholylethylenediamine micro initiator. After that, we conjugated dexamethasone (Dexa) with DOCA-PLL to increase transfection efficiency. |
