| 학회 |
한국공업화학회 |
| 학술대회 |
2007년 봄 (05/11 ~ 05/12, 계명대학교) |
| 권호 |
11권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Purification and Identification of Cancer Markers Using Temperature Controllable Bead Affinity Chromatography Chip Based on MALDI-TOF Analysis |
| 초록 |
Mico-bead affinity chromatography (μ-BAC) has been successfully utilized in purifying and concentrating biologically important proteins in a microchip system. We have fabricated the temperature controllable μ-BAC chip equipped with two separated chambers which contained aptamer-immobilized affinity beads and trypsin-immobilized beads for purifying and identifying the marker proteins. The marker proteins (HCV and AFP) were captured and purified in the first chamber and transferred to the second chamber after thermal releasing, and digested by the immobilized trypsin. Finally, the digested peptides were analyzed and identified by MALDI-TOF. |
| 저자 |
이상명, 윤효진, 이윤식
|
| 소속 |
서울대 |
| 키워드 |
Bead Affinity Chromatography; Microbead; MALDI-TOF; MEMS
|
| E-Mail |
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