| 초록 |
Multi-lamellar protein/liposome complexes, denoted ‘proteolipoplexes’, are self-assembled by multivalent electrostatic association of epidermal growth factor (EGF) with uni-lamellar vesicles (liposomes) of 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP). Anionic EGF (pI = 4.6) mediates the membrane fusion of the cationic DOTAP liposomes with a layer-by-layer process, resulting in protein-intercalated multi-lamellar proteolipoplexes. The EGF/DOTAP complexes exhibit the average diameter of 100-200 nm, loading efficiency of 95 %, and dispersion stability for ＞ 100 days. MD simulation, FRET, cryo-TEM, and Langmuir trough analysis are performed for understanding the interactions between EGF and DOTAP liposome. We discuss the possible mechanism of the multi-lamellar structure formation via multivalent electrostatic attractions. |
