| 초록 |
We developed a non-invasive and patch-type glucose sensor by using a hyaluronate-gold nanoparticle/glucose oxidase (HA-AuNP/GOx) complex with high sensitivity and stable direct electron transfer to accurately detect glucose level in sweat. We synthesized the HA-AuNP/GOx complex by chemical binding of end-thiolated hyaluronic acid (HA-SH) to gold nanoparticles (AuNPs) and physical binding of glucose oxidase (GOx) to surface of a hylauronated-gold nanoparticle (HA-AuNP). The sensor coated with the HA-AuNP/GOx complex exhibited slow evaporation speed of sweat (0.16 μm/min), high sensitivity (3 mg/dl), good selectivity. Especially, our sensor has a high stability repeated use (3 d, applied a 0.7 V to sensor for 2 h/d) and enzymatic activity (14 d). In an in vivo test, we confirmed the correlation between commercially-available glucometer and our patch-type glucose sensor when monitoring glucose level in sweat. |
