| 초록 |
With the increasing antibiotic resistance of methicillin-resistant Staphylococcus aureus (MRSA), which causes from mild infections to serious diseases such as sepsis, it is becoming important to discover new targets to treat them. SarA, a global regulator known to regulate the virulence factors (eg. toxin,adhesin), has been noted as one of these targets. Many studies on the regulatory mechanisms of SarA have been performed at the transcriptional level, but not at the proteome and metabolome levels. Thus, using MRSA and its sarA mutant,we investigated proteomic and metabolomic change through multiomics approaches. As a result, we confirmed an expression change mediated by SarA such as two-component system (SaeSR,WalKR), biofilm or/and adhesin related protein (ClfA,Efb,Atl, etc.), and toxin (SelX,LukGH,PSMs, etc.). It is expected that our study can provide insight into the regulatory mechanisms of SarA at the metabolite and protein level. |
