| 초록 |
Electrode-driven metabolic control is an promising strategy for controlling metabolic fluxes by changing the cellular redox state. Here, we report the electrode-driven heterologous metabolic pathway regulation using a model microorganism, E. coli BL21(DE3), expressing glycerol reductive pathway to produce 3-hydroxypropionic acid (NAD+ dependent metabolism) or 1,3-propanediol (NADH dependent metabolism). The potentiostat provided a potential to shift the cellular redox balance through bacteria-electrode interaction. Consequently, 3-hydroxypropionic acid production was elevated in a positive potential (+ 0.7 V). Controversially, increased 1,3-propanediol productivity with a high NADH/NAD+ ratio was determined in the negative potential chamber (- 0.7 V). The result demonstrated the electrode-driven metabolic regulation for an heterologously expressed glycerol reductive pathway in E. coli. |
