| 학회 | 한국공업화학회 |
| 학술대회 | 2022년 봄 (05/11 ~ 05/13, 제주국제컨벤션센터(ICC JEJU)) |
| 권호 | 26권 1호 |
| 발표분야 | 포스터-생물공학 |
| 제목 | Development and characterization of a fully human antibody targeting EP4 signaling |
| 초록 | Prostaglandin E2 (PGE2) is the predominant prostaglandin in solid tumors and promotes tumor growth and metastasis by activating on its receptor, EP1-4. Among them, EP4 promotes migration and invasion in breast cancer. In this study, we developed fully human EP4 antibodies by phage display technique and selected the antibody (Ab5) with the highest binding affinity and specificity through in vitro assays such as enzyme-linked immunosorbent assay (ELISA) and flow cytometry (FACS). Moreover, we found that Ab5 inhibits PGE2-induced migration and invasion in breast cancer cell line, MDA-MB-231, by downregulating signaling molecules related to metastasis. Taken together, our results suggest that Ab5 can be applied as a new therapeutic agent for the treatment of patients with metastatic breast cancer. |
| 저자 | 김가영, 김남혁, 김현정, 유연규, 허균 |
| 소속 | 국민대 |
| 키워드 | PGE2; EP4; breast cancer; metastasis |
