| 초록 |
Surface-enhanced Raman scattering (SERS)-based immunoassay is an efficient tool which can quickly detect even very small concentrations of target molecule. For multiple detection, distinguishability between targets is important. However, existing platforms have a problem with differentiating certain group of substrates like antibody-coated beads from another until significant difference is observed between SERS spectra of targets. To solve this problem, we introduced shape-coded hydrogel as a substrate. The hydrogel was made of poly (ethylene glycol)-diacrylate (PEG-DA). After that, hydrogel particles were decorated with silver nanoparticles. Square particles were coated with 4-MPBA by self-assembly. Circular particles were processed with HS-PEG2K-NH2 and glutaraldehyde. Then anti-HSA solution was added. As a result, SERS-based multiplex immunoassay applying shape-coded hydrogel is capable of specifically detecting target molecules depending on the shape of hydrogel particle. |
