| 초록 |
Methane is a high potential feedstock for industrial biotechnology due to low cost and huge availability. However, until now, the development of biocatalyst using methane have been constrained by the low productivity and limited genetic tools for methanotrophs. In this study, we have developed inducible gene expression systems for Methylomicrobium alcaliphilum 20Z to convert methane to high-value chemical compounds at low pressure and temperature. To compare promoter strength of various promoters in M. alcaliphilum 20Z, we constructed broad host range vectors, pSGM01, pSGM02, and pSGM03, by fusing the plac, ptac, and pT5 with dtomato gene in the IncP-based MEV vector that can be replicated by Methylomicrobium spp, respectively. In addition, we developed the IPTG inducible expression system not yet applied to methanotroph strains by insertion of lac I geneThese tools make M. alcaliphilum 20Z a promising model system for metabolic engineering for industrial biological conversion of methane. |
