| 학회 |
한국화학공학회 |
| 학술대회 |
2019년 가을 (10/23 ~ 10/25, 대전컨벤션센터) |
| 권호 |
25권 2호, p.1505 |
| 발표분야 |
생물화공 (Biochemical Engineering) |
| 제목 |
Microbial single cell trapping using high-throughput microwells for Surface enhanced Raman spectroscopy imaging |
| 초록 |
Metabolic engineering is a powerful technique that can replace the existing chemical synthesis process for obtaining new metabolites or improving conversion rate. This study introduces the application of single cell trapping using microwells and Surface enhanced Raman spectroscopy (SERS) imaging to metabolic engineering. The combination of microwells and SERS imaging enables the real-time detection of various metabolite concentrations in a single cell level, and it can evaluate various mutants simultaneously. We tested microbial single cell trapping on PDMS microwells. Also, SERS signals were detected for various important metabolites such as ATP. The challenge is to lower the detection limit in vivo, but if spectra references of metabolites and single cell trapping tools are established, the efficiency of metabolic engineering can be greatly developed. |
| 저자 |
김성학1, 장지한2, 정광영2, 김유연2, 양정모2, 조숙형2, 나정걸2, 강태욱2, 연영주3, 이진원4
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| 소속 |
1서강대, 2Sogang Univ., 3Gangneung-Wonjo National Univ., 42C1 Gas Refinery R&D Center |
| 키워드 |
생물화학공학 |
| E-Mail |
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| 원문파일 |
초록 보기 |
