| 초록 |
The aim of this study was to develop a new gene carrier composed of chitosan (CS), cystamine (CA), and PEI. We found that CA and PEI-modified chitosan (CSCAP) can deliver effectively gene into nucleus of mammalian cell with glutathione of high level. Binary complex with pDNA and CSCAP was prepared by electrostatic charge interaction under aqueous condition. Its Gelretardation assay showed that pDNA was completely condensed by CSCAP, and protected from DNase. Also, Its transfection was enhanced by proton sponge effect with modified PEI and dissociation of disulfide-bond by glutathione into cell. In addition, its cellular uptake was investigated by fluorescence microscopy and flow cytometry. Moreover, characterization of CSCAP and binary complex was analysed by 1H-NMR, DLS and TEM. Beside, Its cytotoxicity was evaluated by MTT assay. (No. NRF-2016H1D5A1910499). |
