| 초록 |
L-tyrosine is a commercially important compound in various fields. Several attempts to improve microbial production of L-tyrosine have been implemented. Here, we redistributed and optimized the metabolic pathways by precise control of the carbon flux between L-tyrosine biosynthetic pathway and the TCA cycle at the PEP node for maximum L-tyrosine production in E. coli. A synthetic constitutive promoter and 5’-UTR were designed to control at both transcription and translational levels and carbon flux was rebalanced by controlling the expression level of PEP synthetase using UTR Designer. Using this approach, the L-tyrosine productivity of the engineered E. coli strain was increased resulting in 3.0 g/L of L-tyrosine titer, 0.0354 g L-tyrosine/h/g DCW of productivity, and 0.102 g L-tyrosine/g glucose yield. This work demonstrates that fine-tuning of metabolic engineering by 5’-UTR redesign is an effective strategy for increasing the yield in recombinant L-tyrosine production. |
