| 학회 |
한국화학공학회 |
| 학술대회 |
2018년 가을 (10/24 ~ 10/26, 대구 EXCO) |
| 권호 |
24권 2호, p.1495 |
| 발표분야 |
생물화공(Biochemical Engineering) |
| 제목 |
Sensitive multiplexed Immunoassay using SiO2@AuNP with MEF effect in shape-coded hydrogel |
| 초록 |
Metal-Enhanced Fluorescence(MEF) has been widely studied to improve the sensitivity of protein based bioassays. MEF is a well-known technology, wherein at the certain distance between metallic nanoparticle and fluorophores result in fluorescence enhancement. Silica coated Gold nanoparticle is the most promising candidate for the fluorescence biosensing owing to their low toxicity and high enhanced-fluorescence intensity. Furthermore, hydrogel is a soft and elastic 3D polymeric structured material which absorbs water to provide biological enviroment. In our group, AuNps were coated with the various thickness of silica to optimize the MEF effect. We also prepared different shapes of PEG hydrogel microparticles capable of multiplexed suspension immunoassaays. After immobilizing IgG on the surface of the maximum MEF effected SiO2@AuNPs, entrapped in the different shape of hydrogel microparticles. It is expected to utilize for high sensitive multiplexed immunoassay detecting various analytes. |
| 저자 |
이재상1, 민지홍1, 윤병주1, 노소영1, 고원건2
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| 소속 |
1연세대, 2연세대 화공생명공학과 |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
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