| 초록 |
Hydroxyethyl cellulose (HEC), one of the water-soluble cellulose ether is widely used for variety of applications including pharmaceutical productions. In this study, conjugate of low molecular weight polyethyleneimine (PEI, Mw 2000) and HEC was developed for gene delivery system. The cationic hydroxyethylcellulose derivative (HECP2k) was synthesized by reductive amination of PEI to periodate-oxidized HEC. It could form the positively charged nano-particles with pDNA. HECP2k-treated cells showed high viability in both serum-free condition and serum condition due to the biocompatibility of HEC and low cytotoxicity of PEI2k. Transfection efficiency of HECP2k polyplex was high, similar with that of PEI25k in both serum-free and serum condition. Therefore, HECP2k showed a potential for gene delivery system. |
