| 학회 |
한국화학공학회 |
| 학술대회 |
2014년 봄 (04/23 ~ 04/25, 창원컨벤션센터) |
| 권호 |
20권 1호, p.404 |
| 발표분야 |
생물화공 |
| 제목 |
Production of 5-aminovalerate and glutarate using metabolically engineered Escherichia coli |
| 초록 |
Escherichia coli was metabolically engineered for the production of 5-aminovalerate (5AVA) and glutarate. When the recombinant E. coli WL3110 strain harboring the Pseudomonas putida davAB genes encoding delta-aminovaleramidase and lysine 2-monooxygenase was cultured, 3.6 g/L of 5AVA was produced. When the davAB genes were introduced into recombinant E. coli strain XQ56 allowing improved l-lysine synthesis, 0.27 and 0.5 g/L of 5AVA were produced. We further converted 5AVA to glutarate by expression of the P. putida gabTD genes encoding 5AVA aminotransferase and glutarate semialdehyde dehydrogenase. When recombinant E. coli WL3110 strain expressing the davAB and gabTD genes was cultured, 1.7 g/L of glutarate was produced. [This work was supported by the Technology Development Program to Solve Climate Changes on Systems Metabolic Engineering for Biorefineries from the Ministry of Education, Science, and Technology (MEST) through the National Research Foundation of Korea(NRF-2012-C1AAA001-2012M1A2A2026556). Further support by the World Class University program (R32-2008-000-10142-0) of the MEST are appreciated] |
| 저자 |
김동인1, 박시재1, 김은영2, 노 원2, 박혜민1, 오영훈2, 이승환2, 송봉근2, 제갈종건2, 이상엽1
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| 소속 |
1카이스트, 2KRICT |
| 키워드 |
L-lysine; 5-aminovalerate; Glutarate; Escherichia coli; Metabolic engineering
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| E-Mail |
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| 원문파일 |
초록 보기 |
