| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 봄 (05/12 ~ 05/14, 부산 벡스코(BEXCO)) |
| 권호 |
25권 1호 |
| 발표분야 |
[국제협력] 우수외국인연구자세션 |
| 제목 |
Human DNA purification at high-throughput manner using a centrifugal microfluidic disc for rapid forensic sex-typing |
| 초록 |
We develop a centrifugal disc for high-throughput human nucleic acid (NA) purification to perform the human sex-typing of forensic samples. As a NA extraction matrix, Whatman glass filter paper was used, and sophisticated passive valves were installed to prevent the buffers from overflowing into the neighboring chamber during the injection. We created a portable workstation that includes a buffer storage system, a buffer injection system, and a rotating device to run the disk. The in-house portable workstation managed the whole operation automatically. The extraction of genomic DNA from thirty forensic samples took just ten minutes. The human alphoid repeat sequence of the Y-chromosome and the human 18sRNA were targeted using purified genomic DNA in a loop-mediated isothermal amplification (LAMP) reaction for sex-typing. We were able to complete the genetic sex-typing in 30 minutes using a high-throughput centrifugal disc for NA extraction and the LAMP reaction. |
| 저자 |
HAU VAN NGUYEN, VAN DAN NGUYEN, VU MINH PHAN, HIEP VAN NGUYEN, 서태석
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| 소속 |
Kyung Hee Univ. |
| 키워드 |
High-throughput; nucleic acid purification; centrifugal microfluidics; loop-mediated isothermal amplification; sex-typing
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| E-Mail |
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