| 초록 |
In order to improve neovascularization in skin flaps, we developed an exogenous growth factor (GF) delivery platform comprised of coacervate-coated PLGA nanofibers, using a coacervate that is a self-assembled complex of poly(ethylene argininyl aspartate diglyceride) polycation, heparin, and cargo GFs (i.e., VEGF and/or TGF-β3). The coacervate was coated onto a nanofibrous PLGA membrane for co-administration of dual GFs. An in vivo study using a mouse skin flap model demonstrated that implantation of Coa-Dual NF reduced necrosis and enhanced blood perfusion in skin flap areas after 10 days, as compared to any single GF-loaded coacervate/PLGA fiber along with direct administration of the other GF onto the defect site. Moreover, Coa-Dual NFs exhibited a well-composed skin appendage and a significantly higher number of blood vessels. Therefore, it is concluded that Coa-Dual NFs may lead a synergetic effect of dual GFs for reducing necrosis in the random skin flaps. |
