| 초록 |
Spheroids have been widely applied in tissue engineering fields, such as therapeutic treatment, ex vivo tissue modeling, due to increased cell-cell, cell-ECM interactions. However, oxygen and nutrients that diffuse into the spheroids are limited, which leads to the formation of necrotic cores and a sharp decrease of viability. In this study, we applied porous PCL fibers to enhance the diffusion of spheroid by providing a structural framework inside. We fabricated porous electrospun PCL fibers introduced to form spheroids with human adipose-derived stem cells (hADSCs). PF spheroid showed retained size and viability until 7 days compared to cell only and SF spheroid. The improvement of spheroid diffusion using PF was confirmed directly/indirectly through Hoechst staining and H2O2 diffusion, and structurally confirmed that voids were formed inside the spheroids. By using an engineered fiber, it is possible to improve the diffusion of spheroids with an improved therapeutic effect. |
