| 초록 |
Microalgae are considered as feedstocks for advanced biodiesel with higher productivity and better sustainability than conventional energy crops. High lipid contents is one of desired traits of microalgae for improving economic feasibility of algal biodiesel, but many microalgae accumulate other compounds such as carbohydrates. In this study, increasing lipid contents in microalgae by redirecting carbon flux from carbohydrate or cyanophycin synthesis to lipid synthesis with genetic engineering was investigated. In Synechocystis, glycogen and cyanophycin are a main intracellular storage for carbon, which is accumulated in the absence of nitrogen sources. Genes for enzymes involved in cyanophycin or glycogen synthesis were knocked out to block cyanophycin or glycogen synthesis and increase carbon pool for lipid synthesis. The results from this study could be used for further genetic engineering of microalgae to increase fatty acid productivity. |
