| 초록 |
The main issues, related with non-viral delivery systems for small interfering RNAs (siRNAs), are their poor stability and lack of tumor targetability in vivo. To address these demands, we developed gold-installed polyehtylenimine (PEI)/siRNA complexes (GICs) with a corona of PEGylated hyaluronic acid. When GICs were exposed to 50% serum, no significant change in the structural integrity of siRNA was observed for 24h. Both GICs and gold-deficient (PEI)/siRNA complexes (GDCs) were efficiently internalized into CD44+ melanoma cells (B16F10), but seldom taken up by CD44- normal fibroblast cells (NIH3T3). For RFP-expressing B16F10 cells, GICs showed the highest gene silencing efficacy. Moreover, GICs selectively accumulated at tumor sites after systemic administration in tumor-bearing mice, leading to efficient gene silencing effects. In accordance with their high stability and tumor targetability, GICs might have promising potential as the siRNA delivery system for cancer therapy. |
